Although the involvement of Rho proteins in the pathogenesis of vascular diseases is well studied, little is known about the role of their upstream regulators, the Rho guanine nucleotide exchange factors RhoGEFs. Consistent with these observations, balloon injury BI induced p RhoGEF tyrosine phosphorylation in rat common carotid arteries, and siRNA-mediated down-regulation of its levels substantially attenuated BI-induced smooth muscle cell migration and proliferation, resulting in reduced neointima formation.
Migration and proliferation of vascular smooth muscle cells VSMCs 3 occur during vascular development, in response to vascular injury, and during atherogenesis 12. Various molecules, including cytokines, chemokines, and reactive oxygen species, are produced at the site of vascular injury, and they have been demonstrated to be involved in vascular wall remodeling 3— 5. Among these various molecules, monocyte chemotactic protein 1 MCP1 is one of the prominent chemokine produced at the site of vascular injury that possesses both chemotactic and mitogenic activity toward VSMCs 45.
A large body of evidence suggests that MCP1 plays a crucial role in the recruitment of monocytes, memory T cells, and dendritic cells to the sites of tissue injury, suggesting its importance in cell migration 67. On the other hand, its role in cell proliferation appears to be controversial, as a few studies showed that it negatively modulates VSMC replication 8whereas other reports, including our own, reveal that MCP1 enhances both VSMC migration and proliferation in the development of vascular wall remodeling 59— The Rho family of proteins Rho, Rac, and CDC42 plays an important role in the regulation of various cellular functions, including cell migration and proliferation Many studies have also reported their role in various vascular pathologies both in animals and humans 13— Despite these reports, very little is known about the role of these RhoGEFs in vascular wall remodeling.
We have shown previously that MCP1 is produced at sites of vascular injury and is involved in vascular wall remodeling 5. However, we do not know how Rac1 is activated by MCP1 or injury. An equal amount of protein from the same cell extracts was analyzed by Western blotting for total levels using specific antibodies.
Bottom panelequal amounts of protein from the control and each treatment were also immunoprecipitated with anti-p RhoGEF antibodies. The immunocomplexes were immunoblotted with PY20 antibodies, and the blot was reprobed for p RhoGEF for normalization. To find out whether arterial injury also stimulates p RhoGEF, and if so, the potential role of p RhoGEF in vascular wall remodeling, we used a rat carotid artery restenosis model.
Migration of VSMCs from media to intima is a pathological manifestation of restenosis after angioplasty 3. The assumption is that the migrated VSMCs in the intimal region proliferate, forming neointima following angioplasty or vein grafting 23. Consistent with these observations, depletion of p RhoGEF levels also reduced injury-induced neointima formation, as measured by morphometric analysis Fig.
Acommon carotid arteries from the control or the indicated time periods of post-BI rats were dissected out, and tissue extracts were prepared. The tissue extracts containing an equal amount of protein from each group of pooled arteries were immunoprecipitated IP with Tyr P antibodies, and the immunocomplexes were immunoblotted IB for p RhoGEF. Equal amounts of protein from the same cell extracts were analyzed by Western blotting for total Rac1 and RhoA levels using specific antibodies.
Equal amounts of protein from the same cell extracts were also analyzed by Western blotting for total Rac1 levels. The images were captured under a Zeiss Oberver. Z1 fluorescence microscope using an AxioCam MRm camera.123hulu
Equal amounts of protein from the same tissue extracts were analyzed by Western blotting for total Rac1 levels. These results suggest a correlation between tyrosine phosphorylation of p RhoGEF and activation of Rac1.Momentum and impulse word problems worksheet answers
Equal amounts of protein from the control and each treatment were immunoprecipitated IP with phosphotyrosine antibodies, and the immunocomplexes were immunoblotted IB with anti-p RhoGEF antibodies or analyzed by pulldown assay for Rac1 activation using GST-PAK1 beads. Cell extracts were prepared and analyzed for p RhoGEF tyrosine phosphorylation and Rac1 activation as described in A.Abrus precatorius seeds contain one of the most potent toxins known to man.
If the seeds are crushed and then ingested, more serious toxicity, including death, can occur. We present a case of a man who survived an intentional ingestion of crushed Abrus seeds after he was treated with aggressive gastric decontamination and supportive care.
Abrus precatorius is a vine native to India and other tropical and subtropical areas of the world. Since introduction to Florida and the Caribbean, it is now commonly found throughout these areas and in the southern United States.
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The seeds vary in color, from red, black, orange or white with black and white centers Figure 1. While all parts of the plant are toxic, the highest concentrations are found in the seeds.
Abrus contains a potent toxin, abrin, along with smaller concentrations of glycyrrhizin, aric acid, and N-methyltryptophal. Abrin is a toxalbumin, composed of two subunits, an A and B chain covalently linked through a disulfide bridge.
Oral ingestion of whole seeds often does not produce serious illness since the shell protects the toxin from digestion. Chewing, grinding, or drilling the seed disrupts the hard shell, exposing more abrin to the GI tract. Most effects of this toxin are limited to local GI symptoms, as digestive enzymes destroy the toxin and limit systemic absorption. The majority of cases involving abrin are ingestions with whole seeds. These cases often cause few or mild symptoms because the shell remains intact and there is limited toxin exposure.
Only one previous case documents the intentional ingestion of Abrus seeds that have been ground or crushed. Our case also involves a suicide attempt involving ingestion of ground Abrus seeds.Amd osx discord
However, unlike the previous case that led to death, in this case with aggressive GI decontamination and supportive care, the patient survived with no sequelae. A year-old man presented to the emergency department ED with multiple episodes of vomiting and liquid black stools.
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The patient revealed that he had intentionally ingested the powder of 10 ground Abrus precatorius seeds in a suicide attempt approximately 30 minutes prior to ED arrival. The patient had ground up the seeds and mixed the powder with water to make a liquid slurry.WILLY PAUL MSAFI AMEKASIRIKIA PRESIDENT UHURU KENYATTA ,KUPEWA PESA KIDOGO
After he ingested this liquid slurry, he decided that he did not want to go through with the suicide and self-administered 50 grams of activated charcoal orally just prior to presenting to the ED. Cardiac monitoring showed sinus tachycardia without ectopy or arrhythmias.
On physical examination his head, neck, heart, lung, and neurological exams were normal. Abdominal exam revealed normal bowel sounds with diffuse mild tenderness to palpation. Rectal examination revealed black colored stools that were hemocult negative. Intravenous fluid boluses and anti-emetics were administered.
Despite the history of self-administered charcoal, activated charcoal 50 grams were administered orally in the ED. Liver function tests and CBC were normal. Salicylate, acetaminophen, and ethanol levels were within normal limits, and urine drug screen was negative.Napenda mikundu mikubwa. Nani humu ndani ana mkundu mkubwa unaotoa puru? Napenda MaPuru.
Kama unapenda kutiwa mikono mkunduni au vidole, au sextoys, dildo, chupa au matunda nicheki. Napanuwa mikundu dodoma.
Nafira wanawake na majimama dodoma. Napenda mapuru. Kama una mkundu unaotoka nje nitafute Kama unapenda kutiwa mikono, vidole, chupa, sextoys, dildo au matunda mkunduni nicheki.
Nipo dodoma napenda mikundu yenye mashimo makubwa nicheki Jamani boyz aliyeko Dar, aliye na mkundu mtamu msafi na mwenye mvuto kimuonekano, asiwe mnene asiwe, asiwe mkubwa kuzidi miaka 22, asiye na shida ndogo ndogo, anaye jitambua, anaye jikubali, chek me in Natafuta Mwanamke anayependa kufirwa Mkundu.
Awe na Sura Na Mwonekano Mzuri. Nina Mboo Kubwa Na Ndefu. Nina Uwezo Wa Kufira Mkundu zaidi ya masaa 4 bila mboo kulala au kukojoa. Niandikie Email kupitia mfiraji gmail. Kama unapenda kutiwa mikono, vidole,sextoys, dildo, chupa au matunda mkunduni nicheki. Napanuwa na kufira mikundu dar es salaam.Ericsson rru
Nafira wanawake na majimama dar es salaam. Napanuwa mikundu dar.Chupi nyekundu na supu ya kuku. Image copyright AFP Image caption Wanafunzi hutumia mbinu tofuati kupasi mitihani yao Wakati wa mtihani huwa mgumu kwa watu wengi, hususan Asia mashariki ambapo kuna shinikizo kubwa kwa watu kufaulu. Licha ya tofuati za kitamaduni, wanafunzi wote wana itikadi na imani zao, iwapo ni kuimba nyimbo kula chakula maalum au hata kuvaa nguo wanaoiona kuwa na bahati kwao.
Hizi ni baadhi ya mbinu zinazotumika kote Asia na wanafunzi kuhakikisha wanafualu kwa ukubwa:. Migahawa katika vyuo viku Hong Kong huwapa wanafunzi matufaha na vyakula vinavyotengenezwa kutokana na tufaha wakatimithani ikikaribia. Kwahivyo inadhaniwa utapasi mtihani salama," anasema Chong Wang, kutoka Nanjing China.
Iwapo umsahau kuoga na zaidi kuosha nyele katika harakati zako za kutwalii, usiwe na wasiwasi, huko Korea kusini inadhani kuwa mtu akiosha nyele zake anaosha elimu yote kichwani. Mwezi mmoja kabla ya mitihani Hong Kong, wanafunzi hukusanyika kwa shughuli mbalimbali zinazoanza kwa chakula cha jioni kijulikanacho kama ging guo.
Inaaminika shughuli hizo husaidia wanafunzi kupita mitihani kwa alama za juu. Ni muhimu kwa wanafunzi katika chakula hicho kula nyama ya ngurumwe kwa korosho.
Na chakulwa hicho hupatikana katika migahawa ya kichina ambapo Korosho kwa kichina ni neno sawa na neno"kutarajia kupasi ", na "nyama ya nguruwe" ni sawa na "hamu ya kupata alama ya juu".
Supu ya kuku inadhaniwa kuchangamsha ubongo. Wanafunzi wa Malaysia, Hong Kong, Singapore na China hunywa supu ya kuku wanaposoma kujitayarisha kwa mitihani na asubuhi ya kuamkia mtihani wenyewe. Inatakikana kukusaidia kuwa na makini na hunywa kwa wingi na wanafunzi Singapore. Rangi nyekundu inaaminika kwa ukubwa China kuwani rangi ya bahati nzuri. Kwahivyo watu wengi wanaamini ni vizuri kuvaa nguo nyekundu na zaidi chupi ya ndani wakati wa mtihani.
Wakati mtu anapofanikiwa kuna msemo wa Kichina unaosema, "umevaa chupi nyekundu? Lakini Chong Wang anaonya: "baadhi ya watu huepuka kuvaa ranginyekundu wakati wa mtihani kwasababu China alama za kufeli huandikwa kwa rangi nyekundu. Post a Comment. Dar es salaam Tanzani Matoke ya Darasa la Saba yametangazwa hivi punde hivi punde na mwaka huu ufaulu umeongezeka kutoka asilimia Naibu Waziri wa Elimu na Mafunzo ya Ufundi, Phillipo Mulugo, akifafanua jambo kwa waandishi wa habari, Dar es Salaam jana wakati alipokuwa akitangaza matokeo ya mtihani wa darasa la Sabaofisini kwake.Atherosclerosis and restenosis after angioplasty are the major cardiovascular problems particularly in western countries.
Increased arterial smooth muscle cell growth and accumulation of extracellular matrix proteins are associated with the development of these vascular lesions. We believe that a change in the redox state of the arterial wall triggers these pathological events. Therefore, the major interest of our laboratory is to study the regulation and signal transduction mechanisms of redox-sensitive gene expression in vessel wall cells and investigate their role in atherosclerosis and neointima formation using animal models.
The major techniques used in the laboratory are rat carotid artery injury, hind-limb ischemia, oxygen-induced retinopathy, Northern blot analysis, Western blot analysis, nuclear run-on assay, electrophoretic mobility shift assay, 2-D gel electrophoresis, high performance liquid chromatography, tube formation assay, cell migration assay, tissue culture, transfections, immunohistochemistry, Matrigel plug angiogenesis, chromatin immunoprecipitation assay.
Last Published: Sep 27, Rao, PhD. Gadiparthi N. George and Elizabeth Malloy Professor rgadipar uthsc. The major techniques used in the laboratory are rat carotid artery injury, hind-limb ischemia, oxygen-induced retinopathy, Northern blot analysis, Western blot analysis, nuclear run-on assay, electrophoretic mobility shift assay, 2-D gel electrophoresis, high performance liquid chromatography, tube formation assay, cell migration assay, tissue culture, transfections, immunohistochemistry, Matrigel plug angiogenesis, chromatin immunoprecipitation assay, Research Support NIH RO1HL PI: G.
Yellaturu, C. Neeli, I. N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A. Zeng, Z. A potential role for nuclear factor of activated T cells in receptor tyrosine kinase and G protein-coupled receptor agonist-induced cell proliferation. Biochem J. Bhanoori, M. Arachidonic acid activates translation initiation signaling in vascular smooth muscle cells.
Liu, Z. A novel role for nuclear factor of activated T cells in receptor tyrosine kinase and G protein-coupled receptor agonist-induced vascular smooth muscle cell motility. Dronadula, N. Blockade of nuclear factor of activated T cells activation signaling suppresses balloon injury-induced neointima formation in a rat carotid artery model.There is clearly insufficient room for elephants in my Circassian seeds imported from India.
Wayne's Word. Noteworthy Plants. Biology The owner of a magical seed will be granted a wish for each elephant contained inside it. Circassian Seed With 12 Elephants Inside 2.
Magic Seed On U. Circassian seed containing 12 tiny elephants, good for a dozen wishes. Compound pinnate leaves, seed pods and bright red seeds "jumbie beads" of the necklace tree Adenanthera pavonina growing in the U. Virgin Islands. T housands of years ago, goldsmiths of Asia used the red seeds in their scales to weigh precious gems, gold and silver.
A pearl or diamond weighing 20 grains would be roughly equivalent in weight to five shiny Circassian seeds. Circassian seeds are very similar in shape and size, and have a remarkably uniform weight of about four grains.
It takes about four seeds to make one gram and approximately of them to make an ounce. The head of an ordinary straight pin in the lower right corner shows how small these elephants really are.
Shiny red Circassian seeds or "jumbie beads" Adenanthera pavonina were once used by Goldsmiths of Asia as a standard for weighing precious metals and diamonds. Chicken eyes Adenanthera bicoloranother species of Adenanthera native to Sri Lanka. The seeds are similar to Circassian seeds except they have a black spot at one end. Erythrina caffra B. Erythrina sp. Ormosia monosperma D.Seiko protection circuit
Ormosia cruenta E. Rhynchosia sp. Rhynchosia precatoria G. Abrus precatorius I. Adenanthera pavonina J.
Sophora secundiflora.Hapa tutakuwa tunapost picha za kufirana tu. Picha nzuri taarifa za watu ziwe siri! Mimi mhindi nipo Dar, nina umri 32, naishi upanga, natafuta basha mwenye mboo kubwa na mwili mkubwa, napenda kunyonywa mkundu na kufirwa hadi nitoe machozi kwa utam, napenda kunyonya mboo na kukojolewa shahawa mdomoni.
Basha ninayemtafuta awe juu ya miaka 35 sitaki vijana wadogo au wanafunzi. Mfiraji mwenye mboo kubwa na haswa kabila la wakuria, wanajua sana kufira. Mimi ni mdigo ila nawapenda kufirwa na wakuria. Kama yuko mfiraji mwenye mboo kubwa na kujua kufira vizuri anitafute.Tcp latency
Naitwa Mohammed email msengenafirwa gmail. Haya anaetaka kufirwa mpaka mavi yatoke anitafute kwenye napendakufira yahoo. Enyi mfanyao ujahili katika ardhi, mpaka lini mtaendeleza ufisadi wenu huu. Huyo anayesema ni kinyume na maadili aliingia humu kufanya nini?
Natafta dem alie msafi, awe tayari kufirwa pia awe msafi ili nimnyonye mkundu mpaka akojoe bao la kwanza, kama yupo andika ktk email amfoyu gmail. Nitamfira na kumtomba mpaka atoke mavi na akojoe mara 5. Nitamlipa na hela. Jamani mkundu issue,mwanaume au mwanamke anaye enjoy kufirwa,amitafute nusababa gmail.
Nimependa kuona kuna sehemu watu waweza angalau kutoa duku duku lao. Mimi ni mpenzi mkubwa sana wa kufira, ila nafira wanawake tu, na ninawaridhisha vya kutosha hasa Kwa sasa nahitaji mtuy wa kutulia naye ambaye atakuwa akinimuvuzishia huduma hiyo kwa utuvu na nidhamu aliye tayari wasiliana nami kwa njia hii: jumasongoro yahoo. Nitafute rudebee live. Nipo sinza dar es salaam.
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Me natafuta mwanamke mwenye tako kubwa na mkundu wenye mnato nimnyonye mkundu mpk alie kwa utamu! Na nimtombe mkundu mpk ajickie yupo pepon! Km upo dar au mwanza nitafute kwenye email yangu ili niku2mie namba zangu kwangu hela sio tatzo we nitafute nikupe raha mrbigdick yahoo.
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